LOCUS       40924_23779       12196 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB726 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12196)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..2938
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     terminator      2960..3212
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3246..3262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3270..3286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3294..3324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3339..3360)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3396..3648)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3686..4981)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61235.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3686..4981)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5033..5212)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5718..5742)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6263..7051
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7300..7888
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8074..8214)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8558..8752)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8821..9891)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10323..10949)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggc tagcatgact ggtggacagc aaatgggtgc
     2941 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     3001 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     3061 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     3121 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     3181 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa
     3241 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3361 ggctgaatta ggcgcgccta tttctattaa ttcccgatct agtaacatag atgacaccgc
     3421 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
     3481 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
     3541 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
     3601 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcgc
     3661 ctcgagtgac ttctctgatt gcagactatt tataccaacc agcaagaaag tgtcgaagta
     3721 tgaagcagaa ggcgcaggct agagcctgga aaacgagaag atggatgcaa agagactttt
     3781 ctgatttagg accaaacaac ctcaagtaaa cattgacgag tgtcccatct tttgtgccgg
     3841 tcagtaatcc ggtagcagga tcataccttg ggagacgatg gcgcggacat ttcacaatcc
     3901 cagcaagctg aggaagcgat aatagaaggt tcagaacttg aggaagaaag aagatgagga
     3961 gggtttcact gaagtgaccc agaataccaa caactgccat agtcattccg gcgaagactg
     4021 tgtaagtgtc tccaacaaaa actgaagaag gataccaatt gtaagcaagc attgccaagg
     4081 atgtggccat cagcggttga gtaagaaata tcgagaaggc atgagcttgg tgatactcgg
     4141 gatcaacaga tgctccgatt tgcataacgt tgtgtatcag gatagcagcg gcaataacaa
     4201 ctgtttgacc gatttcaagg ccattcagac cagcgtggat gttaatagag tttgtacaaa
     4261 agacagcaag tagccccata tataacttat atatccttcc tagattaagc acttccaagc
     4321 caatataagc aacaagaggt tttggtataa caatagttgt atgaccagca taagccatca
     4381 aaagtggaag ggtagcaaaa gatggcagga caagttttac tctccaaggc acatcaagga
     4441 catcatctac aaatccaagc aaaatcatga agcaaataga tgctagtgct gcattatact
     4501 ccacaagcca attagaatct tcagtgaagt taaaatactg aaatattatc gccacgatca
     4561 aaaagacaat accaacaaca atacccaatg actctggcac tttaatatct ccttgaggcg
     4621 tgcctctttt gttgatatca aaaccaaaca tgttacgtct cagaacgtat ctggcagtaa
     4681 caggaatcat tttgagagtg acgaaaaacc caaccaagct aagccccgcg ttaattagaa
     4741 tcgatcgctt gagttcatta tcaaccttgt agtgaaaaca gagaaggtat agatagagcg
     4801 aacagagaag agtagagatc acgaagatca ctcctaattt cggcggcgcg agacgaaatt
     4861 cctcaccgga agcagaaacc gtctttcggg tcatcttctg ctccgatggc gctgagttgg
     4921 gttccgacgg gtccggtttg ttgggaattg agatcgatga agctctctta cgagctgcca
     4981 tatcttattg ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga
     5041 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
     5101 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     5161 tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt
     5221 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     5281 tctccgctca tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     5341 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt
     5401 cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta
     5461 cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     5521 tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc
     5581 ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa
     5641 attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     5701 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     5761 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     5821 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     5881 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     5941 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     6001 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     6061 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     6121 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     6181 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     6241 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     6301 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     6361 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     6421 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     6481 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     6541 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     6601 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     6661 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     6721 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     6781 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     6841 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     6901 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     6961 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     7021 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     7081 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     7141 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     7201 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     7261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7441 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7921 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7981 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8041 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     8101 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     8161 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     8221 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8281 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8341 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     8401 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     8461 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     8521 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     8581 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     8641 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     8701 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     8761 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     8821 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     8881 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     8941 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     9001 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     9061 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     9121 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     9181 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     9241 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     9301 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     9361 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     9421 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     9481 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     9541 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     9601 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     9661 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     9721 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     9781 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     9841 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     9901 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
     9961 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    10021 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    10081 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    10141 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    10201 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    10261 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    10321 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    10381 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    10441 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    10501 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    10561 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    10621 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    10681 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    10741 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    10801 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    10861 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    10921 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    10981 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    11041 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    11101 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    11161 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    11221 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    11281 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    11341 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    11401 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    11461 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    11521 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    11581 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    11641 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    11701 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    11761 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    11821 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    11881 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    11941 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    12001 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    12061 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    12121 ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
    12181 acgaacggat aaacct
//
